Different culture media affect growth characteristics, surface marker distribution and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells

BMC Musculoskeletal Disorders

Tobias Gotterbarm³  Wiltrud Richter²  Peer Wolfgang Kämmerer¹  Thomas Dreher³  Sebastian Frank³  Babak Moradi³  Sebastien Hagmann³ 

[1] Maxillofacial and Plastic Surgery, University Medical Center, Mainz, Germany;Research Center for Experimental Orthopedics, University Hospital Heidelberg, Heidelberg, Germany;Department of Orthopedics, Trauma Surgery and Spinal Cord Injury, University Hospital Heidelberg, Germany Schlierbacher Landstrasse 200a, 69118 Heidelberg, Germany

关键词: Adipogenic differentiation;    Chondrogenic differentiation;    Osteogenic differentiation;    Surface markers;    Expansion media;    Mesenchymal stromal cells;   

Others  :  1130182
DOI  :  10.1186/1471-2474-14-223

 received in 2013-04-22, accepted in 2013-07-19,  发布年份 2013

【 摘 要 】

Background

Bone marrow-derived mesenchymal stromal cells (BM-MSCs) play an important role in modern tissue engineering, while distinct variations of culture media compositions and supplements have been reported. Because MSCs are heterogeneous regarding their regenerative potential and their surface markers, these parameters were compared in four widely used culture media compositions.

Methods

MSCs were isolated from bone marrow and expanded in four established cell culture media. MSC yield/1000 MNCs, passage time and growth index were observed. In P4, typical MSC surface markers were analysed by fluorescence cytometry. Additionally, chondrogenic, adipogenic and osteogenic differentiation potential were evaluated.

Results

Growth index and P0 cell yield varied importantly between the media. The different expansion media had a significant influence on the expression of CD10, CD90, CD105, CD140b CD146 and STRO-1. While no significant differences were observed regarding osteogenic and adipogenic differentiation, chondrogenic differentiation was superior in medium A as reflected by GAG/DNA content.

Conclusions

The choice of expansion medium can have a significant influence on growth, differentiation potential and surface marker expression of mesenchymal stromal cells, which is of fundamental importance for tissue engineering procedures.

【 授权许可】

   
2013 Hagmann et al.; licensee BioMed Central Ltd.

【 图 表 】

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