Polymeric film of 6-arm-poly(ethylene glycol) amine graphene oxide with poly (ε-caprolactone): Adherence and growth of adipose derived mesenchymal stromal cells culture on rat bladder

5th Nanosafe International Conference on Health and Safety issues related to nanomaterials for a socially responsible approach

物理学;医药卫生;材料科学

Durán, Marcela^1,2 ; Durán, Nelson^1,3,4 ; Luzo, Angela C.M.^5 ; Duarte, Adriana S. S.^5 ; Volpe, Bruno B.^5 ; Ceragioli, Helder J.^6 ; Andrade, Patricia F.^3 ; De Souza, Joel G.^2 ; Fávaro, Wagner J.^1,2

NanoBioss, Institute of Chemistry (IQ), UNICAMP, Campinas

SP, Brazil^1

Urogenital Carcinogenesis: Urogenital and Immunotherapy Lab., Inst. Biology, University of Campinas (UNICAMP), Campinas

SP, Brazil^2

IQ, Biol. Chem. Lab., UNICAMP, Campinas

SP, Brazil^3

Braz. Nanotechnol. Nat. Lab. (LNNano-CNPEM), Campinas

SP, Brazil^4

Mol. Biol. Lab., Hemocenter-Medicine Fac., UNICAMP, Campinas

SP, Brazil^5

Electric Eng. Comput. Fac., UNICAMP, Campinas

SP, Brazil^6

关键词: Adipose derived stem cells;    Differentiation and proliferations;    Durable materials;    Graft rejection;    Mesenchymal stromal cells;    Solvent evaporation;    Urethral stricture;    Urinary bladder;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012035/pdf
DOI  :  10.1088/1742-6596/838/1/012035

来源: IOP

【 摘 要 】

Nanotechnology has been more present in different fields related to health. The need to find a durable material, of easy use, and which does not interfere significantly in the growth and differentiation of stem cells for the construction of a scaffold for use in urologic surgery, with the purpose of reducing infections, regeneration times and even graft rejection during reconstitution in patients with urethral stricture was conducted a broad survey of information about this and came to the consensus of this project: using graphene oxide, a widely studied nanomaterials which has been presenting numerous beneficial results when in contact with the adipose-derived stem cells. Advanced techniques for the growth, differentiation and proliferation of adipose-derived stem cells were used, as well as the characterization of graphene oxide sheets. For this study, it was prepared the graphene oxide/6 ARM-Poly (ethylene glycol) amine films with poly (-caprolactone). The graphene suspension in organic solvent was prepared by using an ultrasonicator bath and subsequently, the film was formed by solvent evaporation. Total characterization of graphene oxide/6 ARM-PEG-amine/ poly (-caprolactone) film was carried out. It was tested growth and adhesion of adipose-derived stem cells on the film, as well as, were verified the histopathological effects of this scaffold when implanted in the urinary bladder to repair the lesion. Our results demonstrated that this scaffold with adipose-derived stem cells enhanced the repair in rat urinary bladder defect model, resulting in a regular bladder. Improved organized muscle bundles and urothelial layer were observed in animals treated with this scaffold with adipose-derived stem cells compared with those treated only suture thread or scaffold. Thus, our biomaterial could be suitable for tissue engineered urinary tract reconstruction.