Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin‐binding neuroregulatory factors

FEBS Letters

Shioi, Junichi²  Sugahara, Kazuyuki¹  Nishimura, Shuji¹  Umehara, Yuko¹  Yamada, Shuhei¹  Robakis, Nikolaos K.² 

[1] Department of Biochemistry, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan;Department of Psychiatry and Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029, USA

关键词: Alzheimer's disease;    Amyloid precursor protein;    Appican;    Chondroitin sulfate;    Heparin-binding growth factor;    AD;    Alzheimer's disease;    Aβ;    amyloid β;    AβPP;    amyloid precursor protein;    CS;    chondroitin sulfate;    GalNAc;    N-acetyl-D-galactosamine;    GlcUA;    D-glucuronic acid;    GAG;    glycosaminoglycan;    HPLC;    high performance liquid chromatography;    HS;    heparan sulfate;    ΔHexUA;    4-deoxy-α-L-threo-hex-4-enepyranosyluronic acid;    MK;    midkine;    PTN;    pleiotrophin;    bFGF;    basic fibroblast growth factor;    HB-EGF;    heparin-binding epidermal growth factor-like growth factor;    PG;    proteoglycan;   

DOI  :  10.1016/S0014-5793(03)01506-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Appican produced by rat C6 glioma cells, the chondroitin sulfate (CS) proteoglycan form of the amyloid precursor protein, contains an E disaccharide, –GlcUA-GalNAc(4,6-O-disulfate)–, in its CS chain. In this study, the appican CS chain from rat C6 glioma cells was shown to specifically bind several growth/differentiation factors including midkine (MK) and pleiotrophin (PTN). In contrast, the appican CS from SH-SY5Y neuroblastoma cells contained no E disaccharide and showed no binding to either MK or PTN. These findings indicate that the E motif is essential in the interaction of the appican CS chain with growth/differentiation factors, and suggest that glial appican may mediate the regulation of neuronal cell adhesion and migration and/or neurite outgrowth.

【 授权许可】

Unknown