Transforming growth factor β mediates increase of mature transmembrane amyloid precursor protein in microglial cells

FEBS Letters

Mönning, Ursula¹  Banati, Richard B.²  Beyreuther, Konrad¹  Masters, Colin L.³  Sandbrink, Rupert¹ 

[1] Center for Molecular Biology Heidelberg, University of Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany;Department of Neuromorphology, Max-Planck-Institute for Psychiatry, D-82452 Planegg-Martinsried, Germany;Department of Pathology, University of Melbourne, Parkville, Victoria 3052, Australia

关键词: Alzheimer's disease;    βA4 amyloid;    Amyloid precursor protein;    TGFβ;    LPS;    Extracellular matrix;   

DOI  :  10.1016/0014-5793(94)80514-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By using the immortalized microglial cell line BV-2, we show that the high expression of theβA4 amyloid precursor protein (APP), its biogenesis and metabolism is modulated by TGF β, a cytokine with immunosuppressive activity, and by the microglia-stimulating agent LPS. TGFβ induces accumulation of cellular mature APP, the putative precursor of the amyloid subunit of Alzheimer's disease. LPS leads to an increase in cellular immature, non-amyloidogenic APP and secretion of also non-amyloidogenic APP fragments. We also demonstrate a functional involvement of ECM molecules in the regulation of microglial APP expression at mRNA and protein level by TGFβ and LPS.

【 授权许可】

Unknown