Interaction between the zinc(II) and the heparin binding site of the Alzheimer's disease βA4 amyloid precursor protein (APP)

FEBS Letters

Multhaup, Gerd¹  Pollwein, Peter¹  Masters, Colin L.²  Bush, Ashley I.¹ 

[1] Center for Molecular Biology Heidelberg-ZMBH, University Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany;Department of Pathology, University of Melbourne, Parkville, Victoria 3052, Australia

关键词: Alzheimer's disease;    Heparin binding;    Zinc binding;    βA4 amyloid;   

DOI  :  10.1016/0014-5793(94)01176-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Alzheimer's disease βA4 amyloid precursor protein (APP) has been suggested to be involved in regulation of cell growth, neurite outgrowth and adhesiveness through binding to heparin sulfate proteoglycans. In order to unravel the molecular mechanisms underlying those functions in vitro we show that APP binds in a time dependent and saturable manner to the glycosaminoglycan side-chains of proteoglycans but not to chondroitinsulfate. We also demonstrate an interaction between the high affinity heparin binding site within the carbohydrate domain of APP and the zinc(II) binding site of APP. We show that the affinity for heparin is increased two- to four-fold in the presence of micromolar zinc(II). Thus micromolar concentrations of zinc(II) appear to be able to modulate the binding of APP to heparin side-chains of proteoglycans and as shown previously [Science 265 (1994) 1464–1467] to induce the aggregation of soluble amyloid βA4 protein.

【 授权许可】

Unknown