The V‐PLC3 gene encodes a putative plasma membrane‐localized phosphoinositide‐specific phospholipase C whose expression is induced by abiotic stress in mung bean (Vigna radiata L.)

FEBS Letters

Kim, Woo Taek⁴  Bahk, Young Yil²  Hwang, Inhwan³  Lee, Jae-Hoon⁴  Lee, Myoung Hui³  Kim, Jee Eun⁴  Hwang, Byung Kook¹  Kim, Yun Ju⁴  Jung, Ho Won¹ 

[1] Laboratory of Molecular Plant Pathology, College of Life and Environment Science, Korea University, Seoul 136-701, South Korea;Protein Network Research Center, Yonsei University, Seoul 120-749, South Korea;Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, South Korea;Department of Biology, College of Science, Yonsei University, Seoul 120-749, South Korea

关键词: Abiotic stress;    Calcium;    Differential gene expression;    Inositol 1;    4;    5-trisphosphate;    Phospholipase C;    Plasma membrane;    Vigna radiata;    IP3;    inositol 1;    4;    5-trisphosphate;    PCR;    polymerase chain reaction;    PI;    phosphoinositide;    PIP;    phosphatidylinositol 4-phosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PIP5K;    phosphatidylinositol 4-phosphate 5-kinase;    PLC;    phospholipase C;    PI-PLC;    phosphoinositide-specific phospholipase C;    Vr-PLC;    Vigna radiata phospholipase C;   

DOI  :  10.1016/S0014-5793(03)01388-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphoinositide-specific phospholipase C (PI-PLC) catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate inositol 1,4,5-trisphosphate and diacylglycerol, both of which act as secondary messengers in animal cells. In this report, we identified in Vigna radiata L. (mung bean) three distinct partial cDNAs (pVr-PLC1, pVr-PLC2, and pVr-PLC3), which encode forms of putative PI-PLC. All three Vr-PLC genes were transcriptionally active and displayed unique patterns of expression. The Vr-PLC1 and Vr-PLC2 transcripts were constitutively expressed to varying degrees in every tissue of mung bean plants examined. In contrast, the Vr-PLC3 mRNA level was very low under normal growth conditions and was rapidly induced in an abscisic acid-independent manner under environmental stress conditions (drought and high salinity). An isolated genomic clone, about 8.2 kb in length, showed that Vr-PLC1 and Vr-PLC3 are in tandem array in the mung bean genome. The predicted primary sequence of Vr-PLC3 (M _r=67.4 kDa) is reminiscent of the δ-isoform of animal enzymes which contain core sequences found in typical PI-PLCs, such as the catalytic domain comprising X and Y motifs, a lipid-binding C2 domain, and the less conserved EF-hand domain. Results of in vivo targeting experiment using a green fluorescent protein (GFP) showed that the GFP-Vr-PLC3 fusion protein was localized primarily to the plasma membrane of the Arabidopsis protoplast. The C2 domain was essential for Vr-PLC3 to be targeted to the plasma membrane. The possible biological functions of stress-responsive Vr-PLC3 in mung bean plants are discussed.

【 授权许可】

Unknown