CDC25‐dependent induction of inositol 1,4,5‐trisphosphate and diacylglycerol in Saccharomyces cerevisiae by nitrogen

FEBS Letters

Küntzel, Hans¹  Schomerus, Christof¹ 

[1] Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Str. 3, D-3400 Göttingen, Germany

关键词: Saccharomyces cerevisiae;    Inositol 1;    4;    5-trisphosphate;    Diacyl glycerol;    CDC25;    Nitrogen signalling;    Phospholipase C;   

DOI  :  10.1016/0014-5793(92)80688-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of ammonium sulfate to starved yeast cells leads to a 3- to 4-fold rapid increase of the second messengers inositol 1,4,5-trisphosphate (IP₃) and diacylglycerol (DAG), the products of phosphoinositide-specific phospholipase C (PI-PLC). This response is reduced by dissecting the RAS-activating Cdc25 protein, and is completely abolished by the cdc25-1 mutation even at permissive temperature. Starved cdc25-1 mutant cells have a strongly reduced IP₃ content, but an at least 10-fold increased DAG level compared to the isogenic wild-type strain. NH₄ does not stimulate cAMP synthesis, and glucose does not induce IP₃ and DAG. Our data suggest that the Cdc25 protein controls a nitrogen-specific signalling pathway involving the effector PI-PLC, in addition to the glucose-induced activation of adenylyl cyclase (AC).

【 授权许可】

Unknown