Interaction between an intraluminal loop peptide of the inositol 1,4,5‐trisphosphate receptor and the near N‐terminal peptide of chromogranin A

FEBS Letters

Lewis, Marc S²  Yoo, Seung Hyun¹ 

[1] Secretory Granule Research Group, Biomedical Research Center, Korea Advanced Institute of Science and Technology, 373-1 Gusong-dong, Yusong-gu, Daejon 305-701, South Korea;Biomedical Engineering and Instrumentation Program, NCRR, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Chromogranin A;    Inositol 1;    4;    5-triphosphate receptor;    N-terminal peptide;   

DOI  :  10.1016/S0014-5793(98)00393-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The near N-terminal region of chromogranin A (CGA) has been shown to be the secretory vesicle membrane binding region, and tetrameric chromogranin A has been demonstrated to bind four molecules of an intraluminal loop peptide of the inositol 1,4,5-trisphosphate (IP₃) receptor. It was therefore necessary to determine whether the conserved near N-terminal region of CGA interacts with the intraluminal loop region of the IP₃ receptor. In the present study, we found that the proposed anchor region of CGA, the conserved near N-terminal region, does indeed interact with the intraluminal loop region of the IP₃ receptor at the intravesicular pH of 5.5, further strengthening the case for the potential interaction between tetrameric chromogranins and tetrameric IP₃ receptors in the cell.

【 授权许可】

Unknown