Tissue‐specific alternative splicing of mouse brain type ryanodine receptor/calcium release channel mRNA

FEBS Letters

Ichikawa, Yoshiyuki¹  Suwaki, Hiroshi²  Miyatake, Ryosuke²  Iwahashi, Kazuhiko²  Furukawa, Aizo¹  Nakamura, Kazuhiko²  Matsushita, Masayuki² 

[1] Department of Biochemistry, Kagawa Medical University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan;Department of Neuropsychiatry, Kagawa Medical University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-07, Japan

关键词: Ryanodine receptor;    Calcium release channel;    Mouse brain;    Alternative splicing;    RT-PCR;    RyRs;    ryanodine receptors;    RT-PCR;    reverse transcription-polymerase chain reaction;    IP3R;    inositol 1;    4;    5-triphosphate receptor;    CICR;    Ca2+-induced Ca2+release;    E;    embryonic day;    NB;    newborn;   

DOI  :  10.1016/0014-5793(96)01022-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We detected alternative splicing of the mouse brain type ryanodine receptor (RyR3) mRNA. The splicing variant was located in the transmembrane segment. The non-splicing type (RyR3-II) included a stretch of 341 bp, and that of the 13th codon was stop codon TAA. Reverse transcription-polymerase chain reaction (RT-PCR) analysis shows that RyR3-II mRNA was expressed in various peripheral tissues and brain at all developmental stages. However, interestingly, the splicing type (RyR3-I) mRNA was detected only in the cerebrum. These findings suggest that the splicing variants RyR3-I and RyR3-II may generate functional differences of RyR3 in a tissue-specific manner.

【 授权许可】

Unknown