cDNA cloning and sequencing of the human ryanodine receptor type 3 (RYR3) reveals a novel alternative splice site in the RYR3 gene

FEBS Letters

Brenig, Bertram¹  Leeb, Tosso¹ 

[1] Institute of Veterinary Medicine, University of Göttingen, Groner Landstr. 2, 37073 Göttingen, Germany

关键词: Ryanodine receptor type 3;    Ryanodine receptor;    Calcium release channel;    Sequence;    Alternative splicing;    Human fetal brain;   

DOI  :  10.1016/S0014-5793(98)00124-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human ryanodine receptor type 3 (RYR3) was cloned from a fetal brain cDNA library and its complete sequence was determined (EMBL accession number AJ001515). The sequenced cDNA spanned 15 564 bp and contained an open reading frame of 14 613 bp. The corresponding protein consisted of 4870 amino acids with a calculated molecular mass of 552 kDa. Amino acid sequence identities to the RYR3 proteins from rabbit, mink, and chicken were 96%, 95%, and 83% respectively. A previously unidentified alternative splice site was detected generating a transcript that lacked bases 11 569–11 650 and encoded a truncated protein.

【 授权许可】

Unknown