Molecular cloning and characterization of a human brain ryanodine receptor

FEBS Letters

Allen, Paul D.¹  Imoto, Keiji⁴  Nakai, Junichi⁴  Hakamata, Yasuhiro²  Kita, Toru²  Nakashima, Yasuyo²  Maeda, Akito³  Barsoumian, Edward L.³  Nishimura, Seiichiro² 

[1] Department of Anesthesiology, Brigham and Women's Hospital, Boston, MA 02115, USA;Department of Geriatric Medicine, Kyoto University Graduate School, 54 Shogoinkawahara-cho, Sakyo, Kyoto 606-01, Japan;Department of Molecular and Cellular Biology, Nippon Boehringer Ingelheim Co., Ltd., Yato, Kawanishi 666-01, Japan;Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444, Japan

关键词: Ryanodine receptor;    Calcium release channel;    cDNA cloning;    RNA blot hybridization;    Chimeric RyR;    Functional expression;    VGCC;    voltage-gated calcium channel;    RyR;    ryanodine receptor;    MH;    malignant hyperthermia;    PCR;    polymerase chain reaction;    RT-PCR;    reverse transcriptase PCR;   

DOI  :  10.1016/S0014-5793(97)01275-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and sequenced the cDNA of the human brain ryanodine receptor (RyR3), which is composed of 4866 amino acids and shares characteristic structural features with the rabbit RyR3. Northern blot analysis shows that the human RyR3 mRNA is abundantly expressed in hippocampus, caudate nucleus and amygdala as well as in skeletal muscle. The human RyR3 mRNA is also detected in several cell lines derived from human brain tumors. Functional expression of RyR3 and a chimeric RyR suggests that RyR3 forms a calcium-release channel with a very low Ca²⁺ sensitivity.

【 授权许可】

Unknown