Primary structure and functional expression from cDN A of the cardiac ryanodine receptor/calcium release channel

FEBS Letters

Takeshima, Hiroshi¹  Imagawa, Toshiaki¹  Nakai, Junichi¹  Hakamata, Yasuhiro¹  Numa, Shosaku¹  Shigekawa, Munekazu² 

[1] Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan;Department of Molecular Physiology, National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan

关键词: Ryanodine receptor;    Calcium release channel;    cDNA cloning;    cDNA expression;    RNA blot hybridization analysis;    Rabbit heart;    SR;    sarcoplasmic reticulum;    T-tubule;    transverse tubule;    E-C coupling;    excitation-contraction coupling;    IP3;    inositol 1;    4;    5-trisphosphate;   

DOI  :  10.1016/0014-5793(90)80399-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sequence of 4968 (or 4976 with an insertion) amino acids composing the ryanodine receptor from rabbit cardiac sarcoplasmic reticulum has been deduced by cloning and sequencing the cDNA. This protein is homologous in amino acid sequence and shares characteristic structural features with the skeletal muscle ryanodine receptor. Xenopus oocytes injected with mRNA derived from the cardiac ryanodine receptor cDNA exhibit Ca²⁺-dependent Cl⁻ current in response to caffeine, which indicates the formation of functional calcium release channels. RNA blot hybridization analysis with a probe specific for the cardiac ryanodine receptor mRNA shows that the stomach and brain contain a hybridizable RNA species with a size similar to that of the cardiac mRNA. This result, in conjunction with cloning and analysis of partial cDNA sequences, suggests that the brain contains a cardiac type of ryanodine receptor mRNA.

【 授权许可】

Unknown