Isolation and characterization of a gene for a ryanodine receptor/calcium release channel in Drosophila melanogaster

FEBS Letters

Hosoya, Toshihiko¹  Takeshima, Hiroshi²  Hotta, Yoshiki¹  Masai, Ichiro⁴  Miyata, Takashi³  Iwabe, Naoyuki³  Nishi, Miyuki² 

[1] Department of Physics, Faculty of Science, Tokyo University, Tokyo 113, Japan;International Institute for Advanced Studies, Shimadzu N-80, 1 Nishinokyo-Kuwahara-cho, Kyoto 604, Japan;Department of Biophysics, Kyoto University Faculty of Science, Kyoto 606-01, Japan;Molecular Genetics Research Laboratory, Tokyo University, Tokyo 113, Japan

关键词: Ryanodine receptor;    Calcium release channel;    Drosophila;    RNA blot hybridization;    Chromosomal mapping;    IP3;    inositol 1;    4;    5-trisphosphate;    PCR;    polymerase chain reaction;    ER;    endoplasmic reticulum;   

DOI  :  10.1016/0014-5793(94)80634-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of a 25.7 kilobase Drosophila melanogaster genomic DNA segment containing a gene for a ryanodine receptor/calcium release channel homologue has been determined. Computer analysis and partial cDNA cloning revealed 26 exons comprising the protein-coding sequence in this gene. The predicted protein is homologous in amino acid sequence and shares characteristic structural features with the mammalian ryanodine receptors. In blot hybridization analysis, a ~16 kilobase RNA species was identified abundantly in a 6–12 h embryo as the transcript from this gene. In situ hybridization to polytene chromosomes indicated that this gene locates at band position 44F on the second chromosome.

【 授权许可】

Unknown