Ganglioside‐dependent cell attachment and endocytosis of murine polyomavirus‐like particles

FEBS Letters

Lilie, Hauke²  Smith, Alicia E¹  Helenius, Ari¹ 

[1] Institute of Biochemistry, Swiss Federal Institute of Technology-Zurich, CH-8093 Zurich, Switzerland;Institute für Biotechnologie, Martin-Luther-Universität, D-06120 Halle, Germany

关键词: Polyomavirus-like particle;    Ganglioside;    Endocytosis;    Animal virus entry;   

DOI  :  10.1016/S0014-5793(03)01220-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

For murine polyomavirus (Py), previous studies suggest the cellular target is a terminal α2,3-linked sialic acid. Here, we investigate the binding and uptake of mouse polyomavirus-like particles (PyVLP) derived from bacterially expressed VP1. We find that in fibroblast 3T6 cells, binding of PyVLP was substantially reduced by sialidase treatment, but only moderately affected by protease treatment, suggesting glycolipids such as the sialic acid-containing gangliosides mediate cell attachment. We further tested the entry requirement of PyVLP using the ganglioside-deficient GM95 murine cell line, and find PyVLP binding and entry were reduced in these cells. Finally, we find that addition of gangliosides G_M1, G_D1a, and G_T1b to GM95 cells restored cellular PyVLP binding and uptake. Taken together, results indicate that gangliosides function in PyVLP cell attachment and endocytosis.

【 授权许可】

Unknown