Surface chemistry of carbon nanoparticles functionally select their uptake in various stages of cancer cells

Personalized medicine;Endocytosis;Surface chemistry;Carbon nanoparticles

Srivastava, Indrajit ; Pan ; Dipanjan

关键词: Personalized medicine;    Endocytosis;    Surface chemistry;    Carbon nanoparticles;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/97709/SRIVASTAVA-THESIS-2017.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Relationship of the surface physicochemical characteristics of nanoparticles with their interactions with biological entities may provide critical information for nanomedicinal applications. In this work, we have presented the systematic synthesis of sub 50nm carbon nanoparticles (CNP) presenting neutral, anionic, and cationic surface headgroups. A subset of CNPs with ~ 10, 20, and 40nm hydrodynamic sizes are synthesized with neutral surface headgroups.The cellular internalization of these CNPs was systematically quantified for the first time in various stages of breast cancer cells (early, late and metastatic), providing a parametric assessment of charge and size effects. Distinct activities are noticed with these systems as they interact with various stages of the cancer cells. Our results indicated that a metastatic breast cancer could be targeted with a nanosystem presenting anionic phosphate groups. On the contrary, for patients with late stage cancer, drugs could be delivered with sulfonate functionalized carbon nanoparticles with higher probability of intracellular transport. This study will facilitate a better understanding of nanoparticle-biologic interaction and the integration of this knowledge with pathophysiology would help to engineer nanomedicine with superior likelihoods to cross the endocytic “barrier” for delivering drug inside the cancerous cells.