Influence of extracellular matrices on ganglioside pattern of two hepatoma cell lines with different adhesive properties

FEBS Letters

Heidrich, Claudia¹  Geilen, Christoph C.¹  Reutter, Werner¹  Fritsch, Martin¹ 

[1] Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, D-14195 Berlin-Dahlem, Germany

关键词: Ganglioside;    Cell-matrix adhesion;    Extracellular matrix;    Morris hepatoma 7777 cell line;   

DOI  :  10.1016/0014-5793(95)01265-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cell culture model was developed to investigate the involvement of gangliosides in cell-matrix adhesion. Two cell lines with different adhesive properties derived from solid Morris hepatoma 7777 were established. Cultured in horse serum-containing medium, the adhesive cell line (MH 7777_A) adheres and spreads on uncoated culture dishes, whereas the revertant cell line (MH 7777_A>N) does not adhere and grows in suspension. The adhesiveness of both cell lines is dependent on the coating protein used (none, bovine serum albumin, fibronectin or collagen I) and the horse serum concentration in the culture medium. Both cell lines, although of the same origin, differed in their ganglioside composition. The most abundant ganglioside of both MH 777_A and MH 7777_A>N cell lines was fucosyl-G_M1, 0.78 and 0.72 μg per mg cellular protein, respectively. The G_M3 and G_D1a content of MH 7777_A>N cells was significantly higher than that of MH 7777_A cells. Furthermore, a matrix-dependency of the ganglioside pattern of both cell lines was demonstrated.

【 授权许可】

Unknown