Conversion of short‐chain ceramides to short‐chain ceramide GM3 in B16 melanoma cells

FEBS Letters

Ito, Makoto¹  Komori, Hironobu¹ 

[1] Laboratory of Marine Biochemistry, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-81, Japan

关键词: Short-chain ceramide;    C2-ceramide;    C6-ceramide;    Endoglycoceramidase;    Glycosphingolipid;    Ganglioside;    Cer;    ceramide;    GSL(s);    glycosphingolipid(s);    C2-Cer;    N-acetyllsphingosine;    C6-Cer;    N-hexanoylsphingosine;    NBD;    nitrobenzo-2-oxa-1;    3-diazole;    Sph;    sphingosine;    GalCer;    galactosylceramide;    GlcCer;    glucosylceramide;    LacCer;    lactosylceramide;    Gal;    galactose;    EGCase;    endoglycoceramidase;    TLC;    thin-layer chromatography;    PBS;    phosphate buffered saline;    FCS;    fetal calf serum;   

DOI  :  10.1016/0014-5793(95)01137-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report that short-chain ceramide (Cer), C₂- and C₆-Cer, were immediately glycosylated and finally converted to short-chain Cer GM3 in B16 melanoma cells. By addition of either C₂- or C₆-Cer to a cell culture of B16 melanoma in the presence of [¹⁴C]Gal, the radiolabeled precursor, was incorporated into each of two novel glycosphingolipids (GSLs) within 30 min along with synthesis of normal GSLs. These novel GSLs were identified as C₂-, C₆-Cer cerebrosides and C₂-, C₆-Cer GM3, respectively. In comparison with C₂-Cer, C₆-Cer was found to be much more efficiently converted to the GSLs, whereas no glycosylated sphingosine was detectable when it was added in place of short-chain Cer.

【 授权许可】

Unknown