FEBS Letters | |
The CD99 signal enhances Fas‐mediated apoptosis in the human leukemic cell line, Jurkat | |
Jung, Kyeong Cheon3  Kim, Nam Hyun2  Park, Seong Hoe1  Bae, Youngmee2  Park, Weon Seo2  | |
[1] Department of Pathology, College of Medicine, and Research Division for Human Life Science, Seoul National University, Yongon-dong 28, Chongno-gu, Seoul 110-799, South Korea;Department of Pathology, Kangwon National University College of Medicine, 192-1 Hyoja-dong, Chunchon 200-701, Kangwon-do, South Korea;Department of Pathology, Hallym University College of Medicine, 1 Okchon-dong, Chunchon 200-702, Kangwon-do, South Korea | |
关键词: Apoptosis; Caspase; CD99; Epitope; Fas aggregation; 7-AAD; 7-aminoactinomycin D; AMC; aminomethylcoumarin; DiOC6; 3; 3′-dihexyloxacarbocyanine iodide; FasL; Fas ligand; HRP; horseradish peroxidase; 2-ME; 2-mercaptoethanol; mAbs; monoclonal antibodies; PBS; phosphate-buffered saline; Δψ m; mitochondrial membrane potential; | |
DOI : 10.1016/S0014-5793(03)01224-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The CD99 antigen has been implicated in various cellular processes, including apoptosis in T cells. Previously, we reported two monoclonal antibodies that recognize different epitopes of the CD99 molecule, named DN16 and YG32. In this study, we investigated the role of each CD99 epitope in T cell apoptosis. Unlike the DN16 epitope, CD99 ligation via the YG32 epitope failed to induce T cell death. Surprisingly, however, the YG32 signal enhanced Fas-mediated apoptosis in Jurkat T cells. Augmentation of Fas-mediated apoptosis by YG32 ligation was inhibited by treatment with either of the caspase inhibitors z-VAD-fmk or z-IETD-fmk, and YG32 ligation appeared to induce Fas oligomerization. These results suggest that each CD99 epitope plays a distinct role in T cell biology, especially in T cell apoptosis.
【 授权许可】
Unknown
【 预 览 】
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