Homologous p35 proteins of baculoviruses show distinctive anti‐apoptotic activities which correlate with the apoptosis‐inducing activity of each virus

FEBS Letters

Morishima, Nobuhiro²  Okano, Kazuhiro¹  Shibata, Takehiko²  Maeda, Susumu¹ 

[1] Laboratory of Molecular Entomology and Baculovirology, Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198, Japan;Laboratory of Cellular and Molecular Biology, Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

关键词: Apoptosis;    Baculovirus;    p35;    Caspase;    Evolution;   

DOI  :  10.1016/S0014-5793(98)00389-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The anti-apoptotic activity of p35s from two baculoviruses, Autographa californica nucleopolyhedrovirus (AcNPV) and Bombyx mori NPV (BmNPV), was compared in mammalian cells. AcNPV p35 efficiently blocked apoptosis induced by caspase overexpression, but BmNPV p35 did so very poorly. Analysis of chimeric p35s and in vitro cleavage of wild type p35s suggest that the cleavage efficiency of p35 correlates with the blocking activity. Single amino acid substitutions of BmNPV p35 with those observed in AcNPV p35, however, resulted in significant loss of its anti-apoptotic activity. We speculate that sequences flanking the cleavage site have uniquely evolved during baculovirus evolution.

【 授权许可】

Unknown