Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection

BMC Biotechnology

Yiu-Kay Lai²  John T-A Hsu¹  Chih-Chieh Chu¹  Teng-Yuan Chang¹  Kao-Lu Pan¹  Chih-Chien Lin³ 

[1] Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli, 35053, Taiwan, R.O.C

[2] Institute of Biotechnology, Department of Life Science, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan, R.O.C

[3] Department of Cosmetic Science, Providence University, 200 Chung-Chi Road, Shalu, Taichung, 43301, Taiwan, R.O.C

关键词: Sf-caspase-1;    RNA interference;    Chaperone;    Baculovirus;    Apoptosis;   

Others  :  1134672
DOI  :  10.1186/1472-6750-12-83

 received in 2012-06-22, accepted in 2012-11-04,  发布年份 2012

【 摘 要 】

Background

There are few studies that have examined the potential of RNA inference (RNAi) to increase protein production in the baculovirus expression vector system (BEVS). Spodoptera frugiperda (fall armyworm) (Sf)-caspase-1-repressed stable cells exhibit resistance to apoptosis and enhancement of recombinant protein production. However, the mechanism of recombinant protein augmentation in baculovirus-infected Caspase-repressed insect cells has not been elucidated.

Results

In the current study, we utilized RNAi-mediated Sf-caspase-1-repressed stable cells to clarify how the resistance to apoptosis can enhance both intracellular (firefly luciferase) and extracellular (secreted alkaline phosphatase [SEAP]) recombinant protein production in BEVS. Since the expression of molecular chaperones is strongly associated with the maximal production of exogenous proteins in BEVS, the differential expression of molecular chaperones in baculovirus-infected stable cells was also analyzed in this study.

Conclusion

The data indicated that the retention of expression of molecular chaperones in baculovirus-infected Sf-caspase-1-repressed stable cells give the higher recombinant protein accumulation.

【 授权许可】

   
2012 Lai et al.; licensee BioMed Central Ltd.

【 图 表 】

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