Role of iron and ferritin in TNFα‐induced apoptosis in HeLa cells

FEBS Letters

Corsi, Barbara¹  Levi, Sonia¹  Santambrogio, Paolo¹  Gerardi, Gianmario²  Cozzi, Anna¹  Campanella, Alessandro¹  Arosio, Paolo² 

[1] Dibit, Department of Biological and Technological Research, IRCCS H. San Raffaele, Milano, Italy;Department M.T. and Biomedical Technologies, University of Brescia, Via Valsabbina 19, 25125 Brescia, Italy

关键词: Iron regulatory protein;    Ferritin;    Iron metabolism;    Oxidative damage;   

DOI  :  10.1016/S0014-5793(03)00114-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.

【 授权许可】

Unknown