Surface reactivity measurements as required for grouping and read-across: An advanced FRAS protocol

5th Nanosafe International Conference on Health and Safety issues related to nanomaterials for a socially responsible approach

物理学;医药卫生;材料科学

Gandon, Arnaud^1,2 ; Werle, Kai^1 ; Neubauer, Nicole^1 ; Wohlleben, Wendel^1

BASF SE, Dept. Material Physics, Ludwigshafen

67056, Germany^1

Laval University, Department of Chemistry, QC, Canada^2

关键词: Adverse outcomes;    Dose protocols;    Enhanced resolutions;    Oxidative damage;    Particulate Matter;    Physiological condition;    Reporter molecules;    Surface reactivity;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/838/1/012033/pdf
DOI  :  10.1088/1742-6596/838/1/012033

来源: IOP

【 摘 要 】

Oxidative stress is a widely accepted paradigm associated with different adverse outcomes of particulate matter, including nanomaterials. It has frequently been identified in in vitro and in vivo studies and different assays have been developed for this purpose. Here we describe a newly developed multi-dose protocol of the FRAS assay (Ferric Reduction Ability of Serum). The purpose of this SOP is the measurement of the surface reactivity of nanomaterials under physiological conditions. Antioxidative components as present in human blood serum (HBS) serve as reporter molecules. The assay separates the oxidative damage from the read-out of the reporter molecules. The results show significantly enhanced repeatability with better sensitivity towards low reactivity, enabling application of FRAS both to a rough grouping by reactive vs. passive nanomaterials and further to substantiation of read-across by enhanced resolution of the similarity between different nanoforms of the same substance.