The complex relationship between soluble and insoluble tau in tauopathies revealed by efficient dephosphorylation and specific antibodies

FEBS Letters

Revesz, T²  Hanger, D.P⁴  Brion, J.-P³  Anderton, B.H⁴  Lees, A.J¹  de Silva, R¹  Boutajangout, A³  Gibb, G.M⁴ 

[1] Reta Lila Weston Institute of Neurological Studies, Royal Free and University College Medical School, London W1T 4JF, UK;Department of Molecular Pathogenesis, Institute of Neurology, Wakefield Street, London WC1N 1PJ, UK;Laboratory of Histology and Neuropathology, Free University of Brussels, School of Medicine, 808 route de Lennik, Building C-10, CP 601, 1070 Brussels, Belgium;Department of Neuroscience, P.O. Box 38, Institute of Psychiatry KCL, De Crespigny Park, London SE5 8AF, UK

关键词: Tau;    Alzheimer's disease;    Tauopathy;    Neurodegeneration;    Paired helical filament;    Lambda protein phosphatase;    AD;    Alzheimer's disease;    CBD;    corticobasal degeneration;    FTDP-17;    fronto-temporal dementia with Parkinsonism linked to chromosome 17;    PHF-tau;    paired helical filament-tau;    PiD;    Pick's disease;    PSP;    progressive supranuclear palsy;    SDS–PAGE;    sodium dodecyl sulfate–polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(02)03611-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylated tau is deposited as insoluble inclusion bodies in the tauopathies. We have used a new efficient method to dephosphorylate tau extracted from control and tauopathy brain. In some tauopathies, including Alzheimer's disease and progressive supranuclear palsy, the pattern of insoluble tau isoforms reflected that of soluble tau. In contrast, in corticobasal degeneration, Pick's disease, and some forms of fronto-temporal dementia, specific tau isoforms were selectively sequestered into insoluble inclusion-forming tau. Therefore the overall expression of individual tau isoforms does not predict which tau isoforms are deposited in all tauopathies and different mechanisms must operate that result in the deposition of specific tau isoforms.

【 授权许可】

Unknown