A novel striated tropomyosin incorporated into organized myofibrils of cardiomyocytes in cell and organ culture

FEBS Letters

Sanger, Joseph W.¹  Zajdel, Robert W.²  Lee, Sung³  Poiesz, Bernard J.³  Denz, Christopher R.³  Sanger, Jean M.¹  Dube, Syamalima³  Dube, Dipak K.² 

[1] Department of Cell and Developmental Biology, University of Pennsylvania, 421 Curie Blvd., Bldg. BRB23, Philadelphia, PA 19104, USA;Department of Cell and Developmental Biology, Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA;Department of Medicine, Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA

关键词: Smooth muscle tropomyosin;    Green fluorescent protein;    Transfection;    Cardiac myofibril;   

DOI  :  10.1016/S0014-5793(02)02756-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Striated muscle tropomyosin is classically described as consisting of 10 exons, 1a, 2b, 3, 4, 5, 6b, 7, 8, and 9a/b, in both skeletal and cardiac muscle. A novel isoform found in embryonic axolotl heart maintains exon 9a/b of striated muscle but also has a smooth muscle exon 2a instead of exon 2b. Translation and subsequent incorporation into organized myofibrils, with both isoforms, was demonstrated with green fluorescent protein fusion protein construct. Mutant axolotl hearts lack sufficient tropomyosin in the ventricle and this smooth/straited chimeric tropomyosin was sufficient to replace the missing tropomyosin and form organized myofibrils.

【 授权许可】

Unknown