A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre‐mediated recombination

FEBS Letters

Kondoh, Gen³  Miyazaki, Jun-ichi¹  Tashiro, Fumi¹  Niwa, Hitoshi¹  Takeda, Junji³  Sano, Shigetoshi³  Tabayashi, Koichi²  Kawamoto, Shunsuke¹ 

[1] Department of Nutrition and Physiological Chemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;Department of Thoracic and Cardiovascular Surgery, Tohoku University School of Medicine, Sendai, Miyagi 980-8574, Japan;Department of Environmental Medicine, Osaka University Medical School, Suita, Osaka 565-0871, Japan

关键词: Cre/loxP recombination;    Transgenic mouse;    Green fluorescent protein;    β-Actin promoter;    Keratin 5 promoter;    GFP;    green fluorescent protein;    EGFP;    enhanced GFP;    CAT;    chloramphenicol acetyltransferase;    CAG promoter;    modified chicken β-actin promoter with CMV-IE enhancer;    FACS;    fluorescence-activated cell sorter;   

DOI  :  10.1016/S0014-5793(00)01338-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The success of Cre-mediated conditional gene targeting depends on the specificity of Cre recombinase expression in Cre-transgenic mouse lines. As a tool to evaluate the specificity of Cre expression, we developed a reporter transgenic mouse strain that expresses enhanced green fluorescent protein (EGFP) upon Cre-mediated recombination. We demonstrate that the progeny resulting from a cross between this reporter strain and a transgenic strain expressing Cre in zygotes show ubiquitous EGFP fluorescence. This reporter strain should be useful to monitor the Cre expression directed by various promoters in transgenic mice, including mice in which Cre is expressed transiently during embryogenesis under a developmentally regulated promoter.

【 授权许可】

Unknown