Selective binding and magnetic separation of His-tagged proteins using Fe3O4/PAM/NTA-Ni2+ Magnetic Nanoparticles

2017 International Symposium on Application of Materials Science and Energy Materials

材料科学;能源学

Guo, Huiling^1 ; Li, Mengyun^1 ; Tu, Shu^1 ; Sun, Honghao^1

College of Bioengineering and Food, Hunei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan

430068, China^1

关键词: Adsorption efficiency;    External magnetic field;    Fe3O4 nanoparticles;    Green fluorescent protein;    His-tagged proteins;    Magnetic nano-particles;    Magnetic nanoparti cles (MNPs);    Polyacrylamide(PAM);   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/322/2/022017/pdf
DOI  :  10.1088/1757-899X/322/2/022017

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Fe3O4nanoparticles coated with polyacrylamide (PAM) were synthesized. The magnetic core, with an average hydrodynamic size of 235.5 nm, allowed the magnetic nanoparticles (MNPs) rapid separation from solutions under an external magnetic field. NTA-Ni2+was modified on the surface of Fe3O4/PAM MNPs to selectively trap his-tagged green fluorescent protein (GFP). The results showed that Fe3O4/PAM/NTA-Ni2+MNPs exhibited remarkable capability of selective binding and separating his-tagged GFP. The adsorption efficiency was 93.37%.