A rapid and non‐invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP)

FEBS Letters

Nishimune, Yoshitake²  Okabe, Masaru²  Yoshimura, Yasuhide²  Ikawa, Masahito²  Kominami, Katsuya²  Tanaka, Keiichi¹ 

[1] Faculty of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565, Japan;Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565, Japan

关键词: Green fluorescent protein;    Transgenic mouse;    Preimplantation embryo;    β-Actin promoter;    PCR;    Marker;   

DOI  :  10.1016/0014-5793(95)01162-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos. The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken β-actin promoter could be classified as green or non-green under a fluorescent microscope. All the green embryos were shown to carry the transgene by PCR analysis. Taking advantage of the detection of GFP expression can be done non-invasively, the selected embryos were demonstrated to be able to developed to term with 100% of accuracy of the selection.

【 授权许可】

Unknown