Molecular aspects of photodynamic therapy: low energy pre‐sensitization of hypericin‐loaded human endometrial carcinoma cells enhances photo‐tolerance, alters gene expression and affects the cell cycle

FEBS Letters

Palumbo, Giuseppe¹  Maria Veneziani, Bianca¹  Varriale, Linda¹  Coppola, Eva¹  Quarto, Maria¹ 

[1] Dipartimento di Biologia e Patologia Cellulare e Molecolare ‘L. Califano’ and CEOS/CNR, Università di Napoli Federico II, Via S. Pansini 5, 80131 Naples, Italy

关键词: Photodynamic therapy;    Heat shock protein;    Cell cycle;    Apoptosis;    Endometrial carcinoma;   

DOI  :  10.1016/S0014-5793(02)02239-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photosensitization of HEC1-B cells with a low concentration of hypericin and doses of light below 10 J/cm² caused cell death (apoptosis occurred mainly at doses between 2 and 5 J/cm², whereas necrosis prevailed above 6 J/cm²). However, pre-exposure of cells to innocuous irradiation (2 J/cm²) and successive challenge with a light dose that normally induced apoptosis (5 J/cm²) altered the expression of the proteins involved in the regulation of apoptosis, stress response and cell cycle. This change resulted in a significant increase in cell photo-tolerance.

【 授权许可】

Unknown