Hsp70 Expression Profile in Preeclampsia Model of Pregnant Rat (Rattus norvegicus) after Giving the EVOO

1st Annual Applied Science and Engineering Conference

工业技术;自然科学

Irianti, E.^1 ; Ilyas, S.^1 ; Rosidah^1 ; Hutahaean, S.^1

Universitas Sumatera Utara, Indonesia^1

关键词: Antioxidant contents;    Expression levels;    Extra virgin olive oil;    Heat shock protein;    HSP70 expression;    Physical defects;    Simple random sampling;    Treatment group;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/180/1/012161/pdf
DOI  :  10.1088/1757-899X/180/1/012161

来源: IOP

【 摘 要 】

Heat shock protein (Hsp) has long been known to protect cells from oxidative stress. In this case an increased expression is found on several cases of preeclampsia. One of the efforts to prevent preeclampsia is by giving antioxidants such as Extra Virgin Olive Oil (EVOO) or it's better known as olive oil (Oleoa europaea), in the form of extra virgin known for its rich antioxidant content of tocopherols (vitamin E). The purpose of this study is to determine the expression levels of Hsp70 serum on pregnant white rat model of preeclampsia after being given EVOO. This type of research is true experiment; the subjects were female white rats and male virgin with Sprague Dawley, ± 8-11 weeks old, 180g BB s / d 200g, healthy and didn't show any physical defects. Samples were 25 animals, divided into 5 groups, which consisted of different control and treatment given to T2 (rat model of preeclampsia), T3 (rat model of preeclampsia + EVOO 0.45g/bw/day), T4 (rat model of preeclampsia + EVOO 0.9g/bw/day) and T5 (rat model of preeclampsia + EVOO 1.8g/bw/day). The determination of each group was done by simple random sampling. Result on serum levels of Hsp70 that were tested by Elisa test in rats showed the average control was 14.64 mg / ml, group T2: 22:51 mg/ml, T3: 13.62 mg/ml, T4: 15.92 mg/ml, T5: 16:09 mg/ml. ANOVA test showed the P value was 0.001