Cloning and bioinformatics analysis of CcPILS gene of Hickory (Carya cathayensis)

3rd International Conference on Energy Materials and Environment Engineering

能源学;生态环境科学

Guo, Wenbin^1 ; Yuan, Huwei^1 ; Gao, Liuxiao^2 ; Guo, Haipeng^2 ; Qiu, Lingling^1 ; Xu, Dongbin^1 ; Yan, Daoliang^1 ; Zheng, Bingsong^1

State Key Laboratory of Subtropical Silviculture, Zhejiang A and F University, Linan, Hangzhou

311300, China^1

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou

311300, China^2

关键词: Auxin efflux carrier;    Bioinformatics analysis;    Endoplasmic reticulum membranes;    Expression levels;    Grafting process;    Hydrophobic domains;    Real time RT-PCR;    Transmembrane regions;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/61/1/012072/pdf
DOI  :  10.1088/1755-1315/61/1/012072

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

PILS is a key auxin efflux carrier protein in the auxin signal transduction. A CcPILS gene related to hickory (Carya carthayensis) grafting process was obtained by RACE techniques. The full length of CcPILS gene was1541bp contained a 1263bp length open reading flame (ORF). The CcPILS encoded 294 amino acids with molecular weight of 46 kDa, PI 5.38 and localized at endoplasmic reticulum membrane. The gene contained a central hydrophilic loop separating two hydrophobic domains of about five transmembrane regions each. The gene of CcPILS belonged to Clade III sub-family of PILS and its sequence had high homology with Arabidopsis. Real Time RT-PCR analysis showed that the gene expressions were weakly induced in bud, inflorescence, fruit, leaf and stem, while strongly in root. The expression levels were strongly induced and reached a peak at the third day of grafting in scion and rootstock of hickory, which were 1.45 and 3.45 times higher, respectively, compared to that of control. The results indicated that CcPILS may be involved in regulating the expression of genes related to auxin signal transduction during hickory graft process.