Bax‐dependent apoptosis induced by ceramide in HL‐60 cells

FEBS Letters

Choi, Kyung Hee¹  Chun, Young Jin²  Kim, Hae Jong²  Mun, Jung Yee²  Kim, Mie Young² 

[1] Department of Biology, College of Natural Sciences, Chung-Ang University, Seoul 156-756, South Korea;Division of Biochemistry, College of Pharmacy, Chung-Ang University, Seoul 156-756, South Korea

关键词: Ceramide;    Bax;    Bcl-xL;    Apoptosis;    Caspase;    TNF-α;    tumor necrosis factor-α;    zVAD-fmk;    z-Val-Ala-Asp(OMe)-fluoromethyl ketone;    DEVD-pNA;    N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide;    IETD-pNA;    N-acetyl-Ile-Glu-Thr-Asp-p-nitroanilide;    PARP;    poly(ADP-ribose)polymerase;   

DOI  :  10.1016/S0014-5793(01)02836-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ceramide is an important lipid messenger involved in mediating a variety of cell functions including apoptosis. In this study, we show that antisense bax inhibits cytochrome c release, poly(ADP-ribose)polymerase cleavage and cell death induced by ceramide in HL-60 cells. In addition, ceramide induces translocation of Bax to mitochondria. The addition of the broad spectrum caspase inhibitor zVAD-fmk prevented ceramide-induced apoptotic cell death but did not inhibit translocation of Bax and mitochondrial cytochrome c release. Furthermore, ceramide inhibits the expression of the antiapoptotic protein Bcl-xL with an increase in the ratio of Bax to Bcl-xL. These data provide direct evidence that Bax plays an important role in regulating ceramide-induced apoptosis.

【 授权许可】

Unknown