Release of cytochrome c and decrease of cytochrome c oxidase in Bax‐expressing yeast cells, and prevention of these effects by coexpression of Bcl‐xL

FEBS Letters

Guérin, Martine¹  Manon, Stéphen¹  Chaudhuri, Bhabatosh² 

[1] Institut de Biochimie et de Génétique Cellulaire du Centre National de la Recherche Scientifique, Université Victor Ségalen-Bordeaux II, 1 rue Camille Saint-Saëns, F-33077 Bordeaux cedex, France;Oncology Research, Novartis-Pharma, CH-4002 Basel, Switzerland

关键词: Apoptosis;    Bax;    Bcl-xL;    Cytochrome c;    Mitochondrion;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(97)01087-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The characteristics of mitochondria of yeast cells expressing the pro-apoptotic gene Bax or coexpressing Bax and the anti-apoptotic gene Bcl-x_L have been investigated in whole cells, isolated mitochondria and permeabilized spheroplasts. It is found that Bax-induced growth arrest of yeast cells is related to two defects in the respiratory chain: (i) a decrease in the amount of cytochrome c oxidase, the terminal enzyme of the respiratory chain, and (ii) a dramatic increase in the release of cytochrome c to the cytosol. Other components of the inner mitochondrial membrane (bc₁ complex and F₀F₁-ATPase) are unaffected. Coexpression of Bcl-x_L almost fully prevented the effect of Bax. Surprisingly, these results obtained in yeast parallel similar observations reported in mammalian cells.

【 授权许可】

Unknown