Activation of the Permeability Transition Pore by Bax via Inhibition of the Mitochondrial BK Channel

Cellular Physiology and Biochemistry

关键词: Mitochondrial ion channels;    Permeability transition pore;    Mitochondrial BK channel;    Bax;    Bcl-xL;    Apoptosis;   

DOI  :  10.1159/000327944

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Mitochondria are crucially involved in the intrinsic pathway of apoptosis. Upon induction of apoptosis, proapoptotic proteins of the Bcl-2 family, in particular Bax and Bak, transfer the death signal to the organelle. The outcome is release of proapoptotic factors, such as cytochrome c, and mitochondrial changes, such as depolarization. Details of the mechanism by which Bax mediates mitochondrial alterations, however, are unknown. Using the single-channel patch-clamp method, we studied mitoplasts (vesicles of inner membrane) from rat astrocyte and liver mitochondria and intact murine glioma mitochondria to determine the action of proapoptotic Bax and antiapoptotic Bcl-xL on the mitochondrial Ca²⁺-activated channel (mtBK) and the permeability transition pore (mtPTP). Bax (1 nM) inhibited the open probability of the mtBK, whereas Bcl-xL or control proteins had no effect. Incubating mitochondria with iberiotoxin, an inhibitor of mtBK, induced the release of cytochrome c. Bcl-xL inhibited the effects of Bax on mtBK. Furthermore, in patch-clamp studies Bcl-xL inhibited the mtPTP itself, whereas Bax had no direct effect on the mtPTP. We conclude that Bax exerts its proapototic effect by inhibiting mitochondrial K⁺ channels, whereas Bcl-xL exerts its antiapoptotic effect by inhibiting the effects of Bax on mitochondrial potassium channels and by direct inhibition of the mtPTP.

【 授权许可】

CC BY-NC-ND