Phosphatidylserine induces apoptosis in CHO cells without mitochondrial dysfunction in a manner dependent on caspases other than caspases‐1, ‐3, ‐8 and ‐9

FEBS Letters

Yamada, Takeshi¹  Ohyama, Harumi²  Ibuki, Yuko³  Goto, Rensuke³  Miyato, Yasuyuki³ 

[1] Komae Branch, Bio-Science Department, Central Research Institute of Electric Power Industry, Iwado-kita 2-11-1, Komae, Tokyo 201-8511, Japan;Division of Radiobiology and Biodosimetry, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan;Laboratory of Radiation Biology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Shizuoka-shi 422-8526, Japan

关键词: Phosphatidylserine;    Apoptosis;    Caspase dependence;    Mitochondrial transmembrane potential;    Cytochrome c;    Chinese hamster ovary cell;    CHO;    Chinese hamster ovary;    PS;    phosphatidylserine;    ΔΨ;    mitochondrial transmembrane potential;    AMC;    7-amino-4-methylcoumarin;    MCA;    4-methyl-coumaryl-7-amide;    FCM;    flow cytometry;    PI;    propidium iodide;   

DOI  :  10.1016/S0014-5793(01)02771-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, cytochrome c release did not occur in PS-induced apoptosis. A pan caspase inhibitor, Z-VAD, significantly inhibited the apoptosis, but inhibitors of caspase-1, -3, -8 and -9 did not. Activities of caspase-1, -3, -8 and -9 were increased by treatment of the cells with camptothecin, but not with PS. These results suggest that PS-induced apoptosis occurs without the collapse of mitochondrial transmembrane potential and without the release of cytochrome c, in a manner independent of caspase-1, -3, -8 and -9.

【 授权许可】

Unknown