Phosphatidyl serine exposure during apoptosis precedes release of cytochrome c and decrease in mitochondrial transmembrane potential

FEBS Letters

Fiers, Walter¹  Grooten, Johan¹  Vercammen, Dominique¹  Van Loo, Geert¹  Dooms, Hans¹  Vandenabeele, Peter¹  Denecker, Geertrui¹  Declercq, Wim¹ 

[1] Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Apoptosis;    Phosphatidyl serine;    Mitochondrial transmembrane potential;   

DOI  :  10.1016/S0014-5793(99)01702-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Time kinetics of phosphatidyl serine (PS) exposure were compared to other apoptotic parameters following different apoptotic stimuli. Our data indicate that anti-Fas treatment of L929sAhFas cells results in rapid exposure of PS, which precedes decrease in mitochondrial transmembrane potential (ΔΨ _m) and release of cytochrome c, indicating that PS exposure occurs independently of these mitochondrial events. Also during TNF-, etoposide- or staurosporine-mediated apoptosis in PC60 RI/RII cells, PS-positive cells were observed before they had a decreased ΔΨ _m. However, during growth factor depletion-induced death of 32D cells, both phenomena seemed to occur at the same time.

【 授权许可】

Unknown