Radiation‐induced apoptosis in MOLT‐4 cells requires de novo protein synthesis independent of de novo RNA synthesis

FEBS Letters

Taylor, Matthew Hiram¹  Buckwalter, Matthew Ross¹  Taylor, Benjamin James¹  Hart, Janet Leigh¹  O'Neill, Kim Leslie¹  Stephenson, Amen Craig¹ 

[1]Department of Microbiology, Room 751 WIDB, Brigham Young University, University Ave., Provo, UT 84602, USA

关键词: Apoptosis;    Transcription;    Translation;    Ionizing radiation;    Phosphatidylserine;    Mitochondrial transmembrane potential;    IR;    ionizing radiation;    act-D;    actinomycin D;    CHX;    cycloheximide;    Δψ m;    mitochondrial transmembrane potential;    PS;    phosphatidylserine;    FITC;    fluorescein isothiocyanate;    PI;    propidium iodide;   

DOI  :  10.1016/S0014-5793(02)02364-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effects of inhibition of de novo RNA and protein synthesis in ionizing radiation (IR)-induced apoptosis in the human T cell line MOLT-4. We observed that pretreatment with cycloheximide inhibited IR-induced apoptosis. However, pretreatment with actinomycin D did not inhibit apoptosis induced by IR. These results suggest that apoptosis induced by IR in MOLT-4 cells requires de novo protein synthesis but not de novo RNA synthesis. This finding suggests that the mRNA encoding the proapoptotic protein(s) is stabilized to facilitate translation independent of de novo gene transcription in response to IR. Our results also indicate that translation of the required proapoptotic protein(s) occurs upstream of mitochondrial depolarization and after 2 h post-IR.

【 授权许可】

Unknown