Co-occurrence of transcription and translation gene regulatory features underlies coordinated mRNA and protein synthesis

BMC Genomics

Rivka Dikstein²  Edna Schechtman¹  Ana Tamarkin-Ben-Harush² 

[1] Department of Industrial Engineering and Management, Ben Gurion University of the Negev, Beer Sheva 84105, Israel;Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: uORF;    3′UTR;    5′UTR;    uAUG;    Translation;    Transcription;    TATA-less;    TATA-box;   

Others  :  1216262
DOI  :  10.1186/1471-2164-15-688

 received in 2014-04-10, accepted in 2014-08-14,  发布年份 2014

【 摘 要 】

Background

Variability in protein levels is generated through intricate control of the different gene decoding phases. Presently little is known about the links between the various gene expression stages. Here we investigated the relationship between transcription and translation regulatory properties encoded in mammalian genes.

Results

We found that the TATA-box, a core promoter element known to enhance transcriptional output, is associated not only with higher mRNA levels but also with positive translation regulatory features and elevated translation efficiency. Further investigation revealed general association between transcription and translation regulatory trends. Specifically, translation inhibitory features such as the presence of upstream AUG (uAUG) and increased lengths of the 5′UTR, the coding sequence and the 3′UTR, are strongly associated with lower translation as well as lower transcriptional rate.

Conclusions

Our findings reveal that co-occurrence of several gene-encoded transcription and translation regulatory features with the same trend substantially contributes to the final mRNA and protein expression levels and enables their coordination.

【 授权许可】

   
2014 Tamarkin-Ben-Harush et al.; licensee BioMed Central Ltd.

【 图 表 】

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