mik1 + G1–S transcription regulates mitotic entry in fission yeast

FEBS Letters

Anderson, Mark¹  Ng, Szu Shien¹  White, Stewart¹  McInerny, Christopher J¹ 

[1] Division of Biochemistry and Molecular Biology, Institute of Biological and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK

关键词: Transcription;    Cell cycle;    Mitosis;    mik1 +;    Schizosaccharomyces pombe;   

DOI  :  10.1016/S0014-5793(01)02720-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the fission yeast Schizosaccharomyces pombe Mik1p, in combination with Wee1p, is an important inhibitor of mitosis through direct phosphorylation of Cdc2p. Here we present the observation that mik1 ⁺ is transcribed during G1- and S-phase in normally dividing cells. mik1 ⁺ transcription is regulated by the MCB–DSC1 system, which controls expression of other genes at the G1–S interval. mik1 ⁺ is shown to be an important target of MCB–DSC1 as it is epistatic for the mitotic delay phenotype displayed in cdc10-C4 cells, which are mutated in a component of DSC1. The mitotic delay in cdc10-C4 cells is bypassed by cdc2-1w, suggesting that mik1 ⁺ acts directly on cdc2 ⁺, with no checkpoint function involved. Thus, mik1 ⁺ represents a new type of MCB–DSC1 regulated gene in fission yeast, whose gene product is exclusively expressed during G1- and S-phase to prevent premature mitosis during this cell cycle stage.

【 授权许可】

Unknown