Development of affinity technology for isolating individual human chromosomes by third strand binding

Fresco, Jacques R.

Dept. of Molecular Biology, Princeton University (United States)

关键词: Chromosomes;    Plasmids;    Mitosis;    Human Chromosomes;    Nucleic Acids;   

DOI  :  10.2172/820632
RP-ID  :  None
RP-ID  :  FG02-96ER62202
RP-ID  :  820632

美国|英语

来源: UNT Digital Library

【 摘 要 】

The overall goal was to explore whether nucleic acid third strands could be used to bind with very high specificity to specific targets within whole genomes. Towards this end conditions had to be found to keep erroneous binding to an absolute minimum. The goal to use third strands (linked to magnetic beads) to ''capture'' large particles such as plasmids, cosmids, and whole chromosomes from complex mixtures was partially met; their use to serve as cytogenetic probes of metaphase chromosomes and to deliver reactive reagents to unique target sites on chromosomes in vivo for the purpose of mutagenizing specific base pairs was fully met; and their use as cytogenetic probes of chromosomal DNA in sections of formalin-fixed, paraffin-embedded tissue has been met since the DOE support was terminated.