Final progress report, Construction of a genome-wide highly characterized clone resource for genome sequencing

Nierman, William C.

The Institute for Genomic Research (United States)

关键词: Clone Cells;    Neoplasms;    Human Chromosomes;    59 Basic Biological Sciences;    Progress Report;   

DOI  :  10.2172/807742
RP-ID  :  None
RP-ID  :  FC02-97ER62500
RP-ID  :  807742

美国|英语

来源: UNT Digital Library

【 摘 要 】

At TIGR, the human Bacterial Artificial Chromosome (BAC) end sequencing and trimming were with an overall sequencing success rate of 65%. CalTech human BAC libraries A, B, C and D as well as Roswell Park Cancer Institute's library RPCI-11 were used. To date, we have generated >300,000 end sequences from >186,000 human BAC clones with an average read length {approx}460 bp for a total of 141 Mb covering {approx}4.7% of the genome. Over sixty percent of the clones have BAC end sequences (BESs) from both ends representing over five-fold coverage of the genome by the paired-end clones. The average phred Q20 length is {approx}400 bp. This high accuracy makes our BESs match the human finished sequences with an average identity of 99% and a match length of 450 bp, and a frequency of one match per 12.8 kb contig sequence. Our sample tracking has ensured a clone tracking accuracy of >90%, which gives researchers a high confidence in (1) retrieving the right clone from the BA C libraries based on the sequence matches; and (2) building a minimum tiling path of sequence-ready clones across the genome and genome assembly scaffolds.