Protein adducts of the prostate carcinogen PhIP in children

Laboratory, Lawrence Livermore National

Lawrence Livermore National Laboratory

关键词: Amino Acids;    Carcinogens;    Neoplasms;    59 Basic Biological Sciences;    Proteins;   

DOI  :  10.2172/15013945
RP-ID  :  UCRL-TR-202474
RP-ID  :  W-7405-ENG-48
RP-ID  :  15013945

美国|英语

来源: UNT Digital Library

【 摘 要 】

Prostate cancer is the second leading cause of cancer death in men in the United States. few epidemiology studies have indicated that exposure to PhIP, a rodent prostate carcinogen formed in meat during cooking, may be an important risk factor for prostate cancer in humans. Therefore, a highly sensitive biomarker assay is urgently needed to clarify the role of PhIP in prostate cancer. The goal of this project is to develop an assay that can be used to more accurately quantify human exposure to PhIP and potential prostate cancer risk. Our hypothesis is that an Accelerator Mass Spectrometry-based method can be developed to measure protein adducts of PhIP in the blood of humans. This will provide a measure of the internal dose, as well as the capacity for carcinogen bioactivation to a form that can initiate the cancer process. Towards this goal, we have characterized an adduct formed by PhIP in vitro with the amino acid cysteine. This adduct should provide a biomarker of dietary PhIP exposure and potential prostate cancer risk that could be used to identify individuals for prevention and for monitoring the effect chemoprevention strategies.