Molecular cloning of a human polypeptide related to yeast sds22, a regulator of protein phosphatase‐1

FEBS Letters

Wera, Stefaan²  Bollen, Mathieu²  Van Eynde, Aleyde²  Beullens, Monique²  Stalmans, Willy²  Renouf, Sylvie²  Sikela, James¹ 

[1] Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO, USA;Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium

关键词: Cell cycle;    Dephosphorylation;    Mitosis;    Protein phosphatase;    LRR;    leucine-rich repeat;    PP-1;    protein phosphatase-1;    PP-1c;    catalytic subunit of PP-1;    RACE;    rapid amplification of cDNA-ends;   

DOI  :  10.1016/0014-5793(95)01180-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast. We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucinerich repeat structures of 22 residues. Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.

【 授权许可】

Unknown