Increased expression of mitochondrial transcription factor A and nuclear respiratory factor‐1 in skeletal muscle from aged human subjects

FEBS Letters

Fracasso, Flavio¹  Cormio, Antonella¹  Gadaleta, Maria Nicola¹  Lezza, Angela M.S.¹  Vecchiet, Jacopo²  Pesce, Vito¹  Cantatore, Palmiro¹  Felzani, Giorgio² 

[1] Department of Biochemistry and Molecular Biology, University of Bari, and Center of Study on Mitochondria and Energetic Metabolism, CNR, Via Orabona 4, 70125 Bari, Italy;Department of Internal Medicine and Aging, ‘G. D'Annunzio’ University of Chieti, Via dei Vestini, 66013 Chieti Scalo, Italy

关键词: Mitochondrion;    Aging;    Skeletal muscle;    TFAM expression;    NRF-1 expression;   

DOI  :  10.1016/S0014-5793(01)02628-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of two factors involved in the nuclear–mitochondrial crosstalk, namely the mitochondrial transcription factor A (TFAM) and the nuclear respiratory factor-1 (NRF-1), was studied in human skeletal muscle biopsies of young and aged subjects. Aged subjects presented a 2.6-fold and an 11-fold increase of the levels of TFAM protein and TFAM mRNA, respectively. The increased expression of TFAM was associated to the doubling of NRF-1 DNA-binding affinity and to a 6-fold increase of NRF-1 mRNA level. The upregulation of TFAM and NRF-1, in aged skeletal muscle, appears involved in the pathway leading to the age-related increase of mitochondrial DNA content.

【 授权许可】

Unknown