Increase in the adenine nucleotide translocase content of duckling subsarcolemmal mitochondria during cold acclimation

FEBS Letters

Roussel, Damien¹  Chainier, François¹  Rouanet, Jean-Louis¹  Barré, Hervé¹ 

[1] Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS-UCB Lyon 1, Bat. 404, 43 Bd du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France

关键词: Adenine nucleotide translocase;    Cold acclimation;    Skeletal muscle;    Mitochondrion;    ANT;    adenine nucleotide translocase;    CA;    cold-acclimated;    CAT;    carboxyatractyloside;    IFM;    intermyofibrillar mitochondria;    NST;    non-shivering thermogenesis;    SSM;    subsarcolemmal mitochondria;    TN;    thermoneutral control;   

DOI  :  10.1016/S0014-5793(00)01790-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intermyofibrillar and subsarcolemmal mitochondria were isolated from duckling gastrocnemius muscle. The adenine nucleotide translocase (ANT) content of subsarcolemmal mitochondria was found to be half of that present in intermyofibrillar mitochondria. In addition, cold acclimation resulted in a 1.7-fold increase in subsarcolemmal mitochondrial ANT content, with intermyofibrillar mitochondrial ANT remaining constant. This change in mitochondrial ANT content correlates with the previously reported cold-induced change in the sensitivity of mitochondria to palmitate-inhibited ATP synthesis [Roussel et al. (1998) FEBS Lett. 439, 258–262]. It is suggested that the mitochondrial ANT content enhances or reduces the fatty acid uncoupling activity in tissue, depending on the energetic state of mitochondria.

【 授权许可】

Unknown