Dexamethasone treatment specifically increases the basal proton conductance of rat liver mitochondria

FEBS Letters

Roussel, Damien¹  Douay, Olivier¹  Dumas, Jean-François¹  Augeraud, Antoine¹  Malthiéry, Yves¹  Foussard, Françoise¹  Ritz, Patrick¹  Simard, Gilles¹ 

[1] Laboratoire de biochimie et de biologie moléculaire, INSERM EMI-U 00.18, 4 rue Larrey, F-49033 Angers Cedex, France

关键词: Glucocorticoid;    Mitochondrion;    Membrane potential;    Liver;    Skeletal muscle;    IFM;    intermyofibrillar mitochondria;    SSM;    subsarcolemmal mitochondria;    TPMP;    triphenylmethylphosphonium;   

DOI  :  10.1016/S0014-5793(03)00307-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the role that mitochondrial proton leak may play in the glucocorticoid-induced hypermetabolic state. Sprague–Dawley rats were injected with dexamethasone over a period of 5 days. Liver mitochondria and gastrocnemius subsarcolemmal and intermyofibrillar mitochondria were isolated from dexamethasone-treated, pair-fed and control rats. Respiration and membrane potential were measured simultaneously using electrodes sensitive to oxygen and to the potential-dependent probe triphenylmethylphosphonium, respectively. Five days of dexamethasone injection resulted in a marked increase in the basal proton conductance of liver mitochondria, but not in the muscle mitochondrial populations. This effect would have a modest impact on energy expenditure in rats.

【 授权许可】

Unknown