LytB, a novel gene of the 2‐C‐methyl‐D‐erythritol 4‐phosphate pathway of isoprenoid biosynthesis in Escherichia coli

FEBS Letters

Eberl, Matthias¹  Kollas, Ann-Kristin¹  Sanderbrand, Silke²  Altincicek, Boran²  Beck, Ewald¹  Hintz, Martin²  Wiesner, Jochen²  Jomaa, Hassan² 

[1] Biochemisches Institut, Justus-Liebig-Universität, Friedrichstr. 24, 35392 Giessen, Germany;Jomaa Pharmaka GmbH, Frankfurter Str. 50, 35392 Giessen, Germany

关键词: Isoprenoid biosynthesis;    2-C-Methyl-D-erythritol 4-phosphate pathway;    lytB;    DMAPP;    dimethylallyl pyrophosphate;    DOXP;    1-deoxy-D-xylulose 5-phosphate;    IPP;    isopentenyl pyrophosphate;    MEP;    2-C-methyl-D-erythritol 4-phosphate;    MVA;    mevalonate;   

DOI  :  10.1016/S0014-5793(01)02516-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis is essential in many eubacteria, plants, and the malaria parasite. Using genetically engineered Escherichia coli cells able to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway we demonstrate that the lytB gene is involved in the trunk line of the MEP pathway. Cells deleted for the essential lytB gene were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of lytB.

【 授权许可】

Unknown