Identification of (E)‐4‐hydroxy‐3‐methyl‐but‐2‐enyl pyrophosphate as a major activator for human γδ T cells in Escherichia coli

FEBS Letters

Eberl, Matthias²  Kollas, Ann-Kristin²  Altincicek, Boran³  Bahr, Ute⁴  Beck, Ewald²  Reichenberg, Armin³  Hintz, Martin³  Wiesner, Jochen³  Gschwind, Ruth M.¹  Jomaa, Hassan³ 

[1] NMR-Abteilung, Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35043 Marburg, Germany;Biochemisches Institut, Fachbereich Humanmedizin, Justus-Liebig-Universität Giessen, Friedrichstrasse 24, 35392 Giessen, Germany;Jomaa Pharmaka GmbH, Frankfurter Strasse 50, 35392 Giessen, Germany;Instrumentelle Analytische Chemie, Johann Wolfgang Goethe-Universität Frankfurt am Main, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany

关键词: γδ T cell;    2-C-Methyl-D-erythritol 4-phosphate pathway;    4-Hydroxy-3-methyl-but-2-enyl pyrophosphate;    gcpE;    lytB;    DOXP;    1-deoxy-D-xylulose 5-phosphate;    ESI;    electrospray ionization;    FBPP;    3-formyl-1-butyl pyrophosphate;    HMBC;    heteronuclear multiple bond connectivity;    HMB-PP;    (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate;    HMQC;    heteronuclear multi-quantum coherence;    IPP;    isopentenyl pyrophosphate;    MEcPP;    2-C-methyl-D-erythritol 2;    4-cyclopyrophosphate;    MEP;    2-C-methyl-D-erythritol 4-phosphate;    MS;    mass spectrometry;    NMR;    nuclear magnetic resonance;    NOESY;    nuclear Overhauser effect spectroscopy;    TCR;    T cell receptor;   

DOI  :  10.1016/S0014-5793(01)03191-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gcpE and lytB gene products control the terminal steps of isoprenoid biosynthesis via the 2-C-methyl-D-erythritol 4-phosphate pathway in Escherichia coli. In lytB-deficient mutants, a highly immunogenic compound accumulates significantly, compared to wild-type E. coli, but is apparently absent in gcpE-deficient mutants. Here, this compound was purified from E. coli ΔlytB mutants by preparative anion exchange chromatography, and identified by mass spectrometry, ¹H, ¹³C and ³¹P NMR spectroscopy, and NOESY analysis as (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP). HMB-PP is 10⁴ times more potent in activating human Vγ9/Vδ2 T cells than isopentenyl pyrophosphate.

【 授权许可】

Unknown