Functional analysis of the Arabidopsis thaliana GCPE protein involved in plastid isoprenoid biosynthesis

FEBS Letters

Campos, Narciso¹  Querol, Jordi¹  Boronat, Albert¹  Rodrı́guez-Concepción, Manuel¹  Imperial, Santiago¹ 

[1] Departament de Bioquı́mica i Biologia Molecular, Facultat de Quı́mica, Universitat de Barcelona, Martı́ i Franquès 1–7, 08028 Barcelona, Spain

关键词: GCPE;    Isoprenoid biosynthesis;    2-C-Methyl-D-erythritol 4-phosphate pathway;    Plastid targeting;    Arabidopsis;    DXP;    1-deoxy-D-xylulose 5-phosphate;    MEP;    2-C-methyl-D-erythritol 4-phosphate;    CDP-ME;    4-diphosphocytidyl-2-C-methyl-D-erythritol;    ME-cPP;    2-C-methyl-D-erythritol 2;    4-cyclodiphosphate;    IPP;    isopentenyl diphosphate;    DMAPP;    dimethylallyl diphosphate;    MVA;    mevalonic acid;    GcpE;    Escherichia coli gcpE gene product;    GCPE;    Arabidopsis homologue of GcpE;   

DOI  :  10.1016/S0014-5793(02)02402-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plastid isoprenoids are synthesized via the 2-C-methyl-D-erythritol 4-phosphate pathway. A few years after its discovery, most of the Escherichia coli genes involved in the pathway have been identified, including gcpE. In this work, we have identified an Arabidopsis thaliana protein with homology to the product of this gene. The plant polypeptide, GCPE, contains two structural domains that are absent in the E. coli protein: an N-terminal extension and a central domain of 30 kDa. We demonstrate that the N-terminal region targets the Arabidopsis protein to chloroplasts in vivo, consistent with its role in plastid isoprenoid biosynthesis. Although the presence of the internal extra domain may have an effect on activity, the Arabidopsis mature GCPE was able to complement a gcpE-defective E. coli strain, indicating the plant protein is a true functional homologue of the bacterial gcpE gene product.

【 授权许可】

Unknown