Cloning and prokaryotic expression of 1-deoxy-D-xylulose-5-phosphate synthase (DXS) from Lepidium apetalum

2nd International Symposium on Resource Exploration and Environmental Science

生态环境科学

Ma, Ligang^1,2 ; Han, Yongguang^1 ; Zhao, Le^1,2 ; Feng, Weisheng^1,2 ; Kuang, Haixue^3 ; Zheng, Xiaoke^1,2

School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, China^1

Collab. Innov. Ctr. for Resp. Dis. Diagn. and Treatm. and Chinese Med. Development of Henan Province, Zhengzhou, China^2

College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China^3

关键词: 1-deoxy-D-xylulose-5-phosphate synthase;    Full-length cdna;    GenBank;    Isoprenoid biosynthesis;    Methylerythritol phosphate pathways;    PCR amplification;    Prokaryotic expression;    Transcriptome datum;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/170/5/052021/pdf
DOI  :  10.1088/1755-1315/170/5/052021

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

1-Deoxy-D-xylulose-5-phosphate synthase (DXS) is the first enzyme in plastidial methylerythritol phosphate pathway for isoprenoid biosynthesis. With primers designed according to seedling transcriptome data, we cloned a full-length cDNA fragment of DXS gene from Lepidium apetalum by PCR amplification, and designate it as LaDXS (GenBank accession no. KU314760). The gene contains a 2148-bp ORF and encodes a protein with 715 amino acids. Recombinant LaDXS protein was successful expressed in E.coli BL21 (DE3). Cloning and prokaryotic expression of LaDXS is important for further studies of isoprenoid biosynthesis in L. apetalum.