Differential roles of Cassia tora 1-deoxy-D-xylulose-5-phosphate synthase and 1-deoxy-D-xylulose-5-phosphate reductoisomerase in trade-off between plant growth and drought tolerance

Frontiers in Plant Science

Plant Science

Sipei Huang¹  Jiayu Zhou¹  Chaoyue Yan¹  Hai Liao¹  Ruiping Jiang¹  Huige Quan¹  Qiaomu Zheng¹  Guodong Tan¹  Chunyao Tian¹ 

[1] School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, China;

关键词: Cassia tora;    Leguminosae;    isoprenoids;    drought;    trade-off;    1-deoxy-D-xylulose-5-phosphate synthase;    1-deoxy-D-xylulose-5-phosphate reductoisomerase;   

DOI  :  10.3389/fpls.2023.1270396

 received in 2023-07-31, accepted in 2023-10-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

Due to global climate change, drought is emerging as a major threat to plant growth and agricultural productivity. Abscisic acid (ABA) has been implicated in plant drought tolerance, however, its retarding effects on plant growth cannot be ignored. The reactions catalyzed by 1-deoxy-D-xylulose-5-phosphate synthase (DXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) proteins are critical steps within the isoprenoid biosynthesis in plants. Here, five DXS (CtDXS1-5) and two DXR (CtDXR1-2) genes were identified from Cassia tora genome. Based on multiple assays including the phylogeny, cis-acting element, expression pattern, and subcellular localization, CtDXS1 and CtDXR1 genes might be potential candidates controlling the isoprenoid biosynthesis. Intriguingly, CtDXS1 transgenic plants resulted in drought tolerance but retardant growth, while CtDXR1 transgenic plants exhibited both enhanced drought tolerance and increased growth. By comparison of β-carotene, chlorophyll, abscisic acid (ABA) and gibberellin 3 (GA3) contents in wild-type and transgenic plants, the absolute contents and (or) altered GA3/ABA levels were suggested to be responsible for the balance between drought tolerance and plant growth. The transcriptome of CtDXR1 transgenic plants suggested that the transcript levels of key genes, such as DXS, 9-cis-epoxycarotenoid dioxygenases (NCED), ent-kaurene synthase (KS) and etc, involved with chlorophyll, β-carotene, ABA and GA3 biosynthesis were induced and their contents increased accordingly. Collectively, the trade-off effect induced by CtDXR1 was associated with redesigning architecture in phytohormone homeostasis and thus was highlighted for future breeding purposes.

【 授权许可】

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Copyright © 2023 Tian, Quan, Jiang, Zheng, Huang, Tan, Yan, Zhou and Liao